Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages.
نویسندگان
چکیده
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
منابع مشابه
Recovery of primary sporocysts in vivo in the Schistosoma mansoni / Biomphalaria 1 glabrata model using a simple fixation method suitable for extraction of genomic DNA
23 Detailed studies of host/parasite interactions are currently limited because in-situ gene 24 sequencing or monitoring of parasite gene expression is so far limited to genes presenting a 25 high loci copy number in the Schistosome genome or a high level of expression. Indeed, how 26 to investigate the host parasite molecular interplay when parasites are not directly accessible 27 in vivo? Her...
متن کاملRecovery of primary sporocysts in vivo in the Schistosoma mansoni/Biomphalaria glabrata model using a simple fixation method suitable for extraction of genomic DNA and RNA.
Detailed studies of host/parasite interactions are currently limited because in situ gene sequencing or monitoring of parasite gene expression is so far limited to genes presenting a high loci copy number in the Schistosome genome or a high level of expression. Indeed, how to investigate the host parasite molecular interplay when parasites are not directly accessible in vivo? Here we describe a...
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ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 103 5 شماره
صفحات -
تاریخ انتشار 2008